New Gene Submission Form
[contact-form-7 id=”1962″ title=”Gene form”]
Make sure to consider the following levels of evidence/validation for gene functions
Validation | Level of experimental validation |
Hypothetical: only based on similarity to other proteins (e.g. BLAST, Hidden Markov Models /PFAM search). | 1 |
Putative: complete family identification (using phylogenetic trees and including other species) and/or expression data/validation (RNA-seq, qPCR, others). | 2 |
Proposed:correlation experiments such as gene co-expression networks, correlation to metabolite profiles. | 3 |
Candidate: QTL mapping. | 4 |
Validated_other_sp.: If transcription factor: overexpression or negative dominant (in transient, stable experiments) in another species. | 5 |
Validated: knock-out/loss-of-function, silencing, overexpression or negative dominant (transient, stable) in the same species. If enzyme: in vitro/recombinant enzyme characterization (e.g. E. coli/yeast) or overexpression of enzyme (transient, stable) in another species. | 6 |